Cell manipulation, micromanipulation, and microinjection (e.g., using a micromanipulation pipette, a glass capillary, or a micromanipulator)

Cultivation and high-resolution microscopy of cells

Immunofluorescence staining

Widefield and confocal fluorescence microscopy of living and fixed cells

Live cell imaging over extended time periods

Transfection assays

Differential interference contrast (DIC) when using a DIC Lid

Cell location and counting when using the gridded version

Want to know if you should use a glass or a polymer bottom for your application? Find out here.

Specifications

Technical Features

Standard format imaging dish with a 35 mm diameter for tissue culture

Low walls for a large access area during the micromanipulation of cells; also available with high walls with a standard height of 12 mm for easy handling: μ-Dish ^{35 mm, high}

ibiTreat (tissue culture-treated) surface for optimal cell adhesion and cell growth

Lid with locking feature for minimal evaporation

No autofluorescence

Biocompatible polymer material—no glue, no leaking

Also available as μ-Dish ^{35 mm, low} Glass Bottom with a #1.5H Glass Coverslip Bottom, which is specifically suited for TIRF and single-molecule applications

Available with a 500 μm grid: μ-Dish ^{35 mm, low} Grid-500

The Principle of the μ-Dish ^{35 mm, low}

Low Walls for Easy Micromanipulation of Cells

The ibidi μ-Dishes with low walls (i.e., μ-Dish ^{35 mm, low} and μ-Dish ^{35 mm, low} Glass Bottom) are perfectly suited for micromanipulation assays, such as intracellular injections, micropipette chemotaxis assays, or tissue treatment and microscopy. Due to the low walls of these μ-Dishes, instrumentation (e.g., electrodes) can be positioned exactly using a micromanipulator. The excellent optical quality makes the ibidi μ-Dishes ideally suited for single-cell handling and high-resolution live cell imaging. For all standard cell culture applications, we also offer the μ-Dish ^{35 mm, high} product family with high walls.

The Coverslip Bottom

The μ-Dish ^{35 mm, low} comes with a thin ibidi Polymer Coverslip Bottom that has the highest optical quality (comparable to glass) and is ideally suitable for high-resolution microscopy. It is also available as μ-Dish ^{35 mm, low} Glass Bottom with a #1.5H Glass Coverslip Bottom for TIRF or single molecule microscopy.

The ibiTreat Surface

ibiTreat (tissue culture-treated) is our most recommended surface modification, because almost all adherent cells grow well on it without the need for any additional coating.

Learn more about the coverslip bottom or the different surfaces of the ibidi chambers here.

Lid with Locking Feature for Minimized Evaporation

All ibidi μ-Dishes are equipped with a special lid-locking feature. The locking position minimizes evaporation and thereby provides excellent conditions for long-term studies in a non-humidified environment. Gas exchange (carbon dioxide or oxygen) during cell culture is maintained thanks to the gas-permeable polymer material of the dish.

TIP: Use the locking feature if minimal evaporation is required (e.g., outside incubators, non-humidified microscopy stages, etc.).

Comparison of the ibidi Dishes ^{35 mm, low}

Please find a detailed comparison of the material specifications, including suitable microscopy applications for the ibidi Polymer Coverslip, the ibidi Glass Bottom, and further materials, here.

The ibidi μ-Dishes: High Walls vs. Low Walls